Medaka Polishing and Variant Calling

Medaka Polishing and Variant Calling

Basic Consensus Polishing

# Polish assembly with medaka
medaka_consensus -i reads.fastq.gz \
    -d draft_assembly.fa \
    -o medaka_output \
    -t 4 \
    -m r1041_e82_400bps_sup_v5.0.0

Variant Calling (Haploid)

# Call variants against reference (medaka v2.0+)
medaka_variant \
    -i reads.fastq.gz \
    -r reference.fa \
    -o output_dir \
    -m r1041_e82_400bps_sup_v5.0.0

Note: Diploid variant calling has been deprecated in medaka v2.0. For diploid samples, use Clair3 instead.

Step-by-Step Workflow (medaka v2.0+)

# 1. Align reads to reference/​draft
minimap2 -ax map-ont reference.fa reads.fastq.gz | \
    samtools sort -o aligned.bam
samtools index aligned.bam

# 2. Run neural network inference
medaka inference aligned.bam consensus.hdf \
    --model r1041_e82_400bps_sup_v5.0.0 \
    --threads 2                          # >2 threads has poor scaling

# 3. Create consensus sequence from probabilities
medaka sequence consensus.hdf reference.fa polished.fa

# 4. Call variants from probabilities
medaka vcf reference.fa consensus.hdf variants.vcf

List Available Models

# See all available models
medaka tools list_models

# Models are named:
# r{pore}_{chemistry}_{speed}bps_{accuracy}_{version}
# e.g., r1041_e82_400bps_sup_v5.0.0

Common Models

Choose Model Based on Basecaller

# Check which basecaller was used in your data
# Then select matching model

# For Guppy/​Dorado SUP basecalling on R10.4.1
medaka_consensus -m r1041_e82_400bps_sup_v5.0.0 ...

# For HAC basecalling
medaka_consensus -m r1041_e82_400bps_hac_v5.0.0 ...

Polish Region Only

# Polish specific region (medaka v2.0+)
medaka inference aligned.bam consensus.hdf \
    --model r1041_e82_400bps_sup_v5.0.0 \
    --region chr1:1000000-2000000

Multiple Rounds of Polishing

# First round
medaka_consensus -i reads.fastq.gz -d draft.fa -o round1 -m model

# Second round (diminishing returns, usually not needed)
medaka_consensus -i reads.fastq.gz -d round1/​consensus.fasta -o round2 -m model

Call Variants from Existing BAM

# If you already have aligned BAM (medaka v2.0+)
medaka inference aligned.bam consensus.hdf --model r1041_e82_400bps_sup_v5.0.0
medaka vcf reference.fa consensus.hdf variants.vcf

Filter VCF Output

# Filter by quality
bcftools filter -i 'QUAL>20' variants.vcf > variants.filtered.vcf

# Get high-confidence calls
bcftools view -i 'FILTER="PASS"' variants.vcf > variants.pass.vcf

Output Files

Key Parameters

GPU Acceleration

# Enable GPU (if available)
medaka_consensus -i reads.fastq.gz -d draft.fa -o output \
    -m r1041_e82_400bps_sup_v5.0.0 \
    -b 100 \                       # Increase batch size for GPU
    -t 4

Related Skills

• long-read-alignment - Generate input alignments
• structural-variants - Find SVs from polished assembly
• variant-calling/​variant-calling - Short-read variant calling comparison