MarkersFinder Process Configuration

MarkersFinder Process Configuration

Purpose

Flexible marker finding process that wraps Seurat's FindMarkers function for custom group comparisons beyond simple cluster-vs-all analysis. Unlike ClusterMarkers (all-vs-all cluster comparisons), MarkersFinder enables targeted differential expression analysis between specific groups, conditions within cell types, or any custom comparison defined by metadata columns. Automatically performs pathway enrichment analysis on significant markers and generates comprehensive visualizations.

When to Use

• Custom group comparisons: Compare specific clusters (e.g., c1 vs c3)
• Condition effects within cell types: Treatment vs control in T cells
• Targeted differential expression: Focus on biologically meaningful comparisons
• Multi-sample analysis: Find markers across different samples/​batches
• Subset-based comparisons: Compare cell states within defined populations
• Complex experimental designs: Multi-condition, multi-factor comparisons

Note: Use ClusterMarkers for standard all-vs-all cluster analysis. Use MarkersFinder for custom comparisons.

Configuration Structure

Process Enablement

[MarkersFinder]
cache = true

Input Specification

[MarkersFinder.in]
srtobj = ["SeuratClustering"]

Environment Variables

Core Group Specification

[MarkersFinder.envs]
group_by = "seurat_clusters"  # Column in metadata to group cells
ident_1 = ""  # First group (ident.1); if empty, all groups vs rest
ident_2 = ""  # Second group (ident.2); if empty, ident_1 vs rest
each = ""  # Column to create separate cases for each unique value

Statistical Test Selection (Seurat FindMarkers)

[MarkersFinder.envs]
test.use = "wilcox"  # Options: wilcox, MAST, DESeq2, roc, t, tobit, bimod, poisson, negbinom, LR
logfc.threshold = 0.25  # Minimum log2 fold change
min.pct = 0.1  # Minimum percentage of cells expressing gene
min.diff.pct = -Inf  # Minimum difference in detection
only.pos = false  # Only positive markers
min.cells.group = 3  # Minimum cells per group
min.cells.feature = 3  # Minimum cells expressing gene

Significant Markers Filter & Enrichment

[MarkersFinder.envs]
sigmarkers = "p_val_adj < 0.05"  # Filter for enrichment (vars: p_val, avg_log2FC, pct.1, pct.2, p_val_adj)
dbs = ["KEGG_2021_Human", "MSigDB_Hallmark_2020"]  # Pathway databases
enrich_style = "enrichr"  # Options: enrichr, clusterprofiler

Multiple Comparison Cases

[MarkersFinder.envs.cases."T_vs_B"]
group_by = "celltype"
ident_1 = "T cells"
ident_2 = "B cells"

[MarkersFinder.envs.cases."Treatment_vs_Control"]
group_by = "condition"
ident_1 = "treatment"
ident_2 = "control"
subset = "celltype == 'T cells'"

External References

Seurat FindMarkers Parameters

https://satijalab.org/seurat/reference/findmarkers

Key Parameters:

• ident.1, ident.2: Groups to compare
• test.use: Statistical test (wilcox, MAST, DESeq2, roc, etc.)
• logfc.threshold: Minimum fold change (log2 scale)
• min.pct: Minimum percentage of cells expressing gene
• min.diff.pct: Minimum difference in detection between groups
• only.pos: Return only positive markers (higher in ident.1)

Enrichment Databases

• "KEGG_2021_Human", "KEGG": KEGG pathways
• "MSigDB_Hallmark_2020", "Hallmark": MSigDB Hallmark
• "GO_Biological_Process_2025": GO Biological Process
• "Reactome_Pathways_2024", "Reactome": Reactome
• "WikiPathways_2024_Human", "WikiPathways": WikiPathways

Full list: https://maayanlab.cloud/Enrichr/#libraries

Configuration Examples

Minimal Configuration

[MarkersFinder]
[MarkersFinder.in]
srtobj = ["SeuratClustering"]
[MarkersFinder.envs]
group_by = "seurat_clusters"

Cluster-to-Cluster Comparison

[MarkersFinder.envs]
group_by = "seurat_clusters"
ident_1 = "c1"
ident_2 = "c3"
logfc.threshold = 0.25
sigmarkers = "p_val_adj < 0.05 & avg_log2FC > 0"

Condition Comparison within Cell Type

[MarkersFinder.envs]
group_by = "condition"
ident_1 = "treatment"
ident_2 = "control"
subset = "celltype == 'T cells'"
test.use = "MAST"

Multiple Comparison Cases

[MarkersFinder.envs.cases."T_vs_B"]
group_by = "celltype"
ident_1 = "T cells"
ident_2 = "B cells"
[MarkersFinder.envs.cases."CD4_vs_CD8"]
group_by = "subtype"
ident_1 = "CD4+ T"
ident_2 = "CD8+ T"
subset = "celltype == 'T cells'"

Cross-Sample Comparison (Using each)

[MarkersFinder.envs]
group_by = "seurat_clusters"
ident_1 = "c1"
ident_2 = "c2"
each = "Sample"

Common Patterns

Pattern 1: Specific Cluster Pair

[MarkersFinder.envs]
group_by = "seurat_clusters"
ident_1 = "c1"
ident_2 = "c3"
logfc.threshold = 0.25
sigmarkers = "p_val_adj < 0.05 & avg_log2FC > 0"

Pattern 2: Treatment Effect per Cell Type

[MarkersFinder.envs.cases."Treatment_Tcells"]
group_by = "condition"
ident_1 = "treatment"
ident_2 = "control"
subset = "celltype == 'T cells'"
[MarkersFinder.envs.cases."Treatment_Bcells"]
group_by = "condition"
ident_1 = "treatment"
ident_2 = "control"
subset = "celltype == 'B cells'"

Pattern 3: Multiple Contrasts

[MarkersFinder.envs.cases."T_vs_B"]
group_by = "celltype"
ident_1 = "T cells"
ident_2 = "B cells"
[MarkersFinder.envs.cases."CD4_vs_CD8"]
group_by = "subtype"
ident_1 = "CD4+ T"
ident_2 = "CD8+ T"
subset = "celltype == 'T cells'"

Pattern 4: All Clusters vs Rest (Like ClusterMarkers)

[MarkersFinder.envs]
group_by = "seurat_clusters"

Pattern 5: Cross-Batch Comparison with each

[MarkersFinder.envs]
group_by = "seurat_clusters"
ident_1 = "c1"
ident_2 = "c2"
each = "Batch"
overlaps = {"Batch_Overlap": {plot_type = "venn"}}

Difference from ClusterMarkers

When to use which:

• ClusterMarkers: Standard cluster identification, all-vs-all comparisons, quick cluster annotation
• MarkersFinder: Custom comparisons, condition effects, cell type-specific DE, multi-factor designs

Dependencies

• Upstream: SeuratClustering (provides cluster assignments and metadata)
• Downstream: Custom differential expression analysis, cell type annotation
• Related: ClusterMarkers (simpler all-vs-all), PseudoBulkDEG (bulk-like DE)

Validation Rules

• group_by must be valid column in Seurat object metadata
• ident_1 and ident_2 must exist in group_by column if specified
• Groups must have ≥ min.cells.group cells (default: 3)
• Genes must be expressed in ≥ min.cells.feature cells (default: 3)
• For each: creates separate case for each unique value in column
• sigmarkers must be valid R/​dplyr expression with available variables: p_val, avg_log2FC, pct.1, pct.2, p_val_adj

Troubleshooting

Issue: Group Not Found

Symptoms: Error "ident.1 not found" Solution: Verify group_by column name and ident_1/ident_2 values match Seurat object metadata exactly (case-sensitive)

Issue: Insufficient Cells in Groups

Symptoms: No markers found or cell count error Solution: Reduce min.cells.group and min.cells.feature to 1, or combine similar groups via mutaters

Issue: No Significant Markers Found

Symptoms: Empty marker tables Solution: Loosen thresholds: logfc.threshold = 0.1, min.pct = 0.05, sigmarkers = "p_val_adj < 0.1 & avg_log2FC > 0"

Issue: Too Many Markers Found

Symptoms: Thousands of markers, computationally expensive Solution: Tighten thresholds: logfc.threshold = 0.58 (1.5-fold), min.pct = 0.25, sigmarkers = "p_val_adj < 0.01 & avg_log2FC > 1"

Issue: Enrichment Analysis Returns No Results

Symptoms: Empty enrichment tables Solution: Loosen sigmarkers filter and add more databases: dbs = ["KEGG_2021_Human", "MSigDB_Hallmark_2020", "Reactome_Pathways_2024"]