hovernet-histo-cin2-nuc-v1

Hover-Net Model for Cervical Intraepithelial Neoplasia 2 (CIN2) Nuclei Segmentation

Model

• cellseg_models.pytorch implementation of Hover-Net: https://www.sciencedirect.com/science/article/pii/S1361841519301045
• Backbone encoder: pre-trained efficientnet_b5 from pytorch-image-models https://github.com/huggingface/pytorch-image-models

USAGE

1. Install cellseg_models.pytorch and albumentations

pip install cellseg-models-pytorch
pip install albumentations

2. Load trained model

from cellseg_models_pytorch.models.hovernet import HoverNet

model = HoverNet.from_pretrained("cin2_v1_efficientnet_b5")

3. Run inference for one image

from albumentations import Resize, Compose
from cellseg_models_pytorch.utils import FileHandler
from cellseg_models_pytorch.transforms.albu_transforms import MinMaxNormalization

model.set_inference_mode()

# Resize to multiple of 32 of your own choosing
transform = Compose([Resize(1024, 1024), MinMaxNormalization()])

im = FileHandler.read_img(IMG_PATH)
im = transform(image=im)["image"]

prob = model.predict(im)
out = model.post_process(prob)
# out = {"nuc": [(nuc instances (H, W), nuc types (H, W))], "cyto": None, "tissue": None}

3.1 Run inference for image batch

import torch
from cellseg_models_pytorch.utils import FileHandler

model.set_inference_mode()

# dont use random matrices IRL
batch = torch.rand(8, 3, 1024, 1024)

prob = model.predict(im)
out = model.post_process(prob)
# out = {
#  "nuc": [
#    (nuc instances (H, W), nuc types (H, W)),
#    (nuc instances (H, W), nuc types (H, W)),
#    .
#    .
#    .
#    (nuc instances (H, W), nuc types (H, W))    
#  ],
#  "cyto": None,
#  "tissue": None
#}

4. Visualize output

from matplotlib import pyplot as plt
from skimage.color import label2rgb

fig, ax = plt.subplots(1, 3, figsize=(18, 6))
ax[0].imshow(im)
ax[1].imshow(label2rgb(out["nuc"][0][0], bg_label=0)) # inst_map
ax[2].imshow(label2rgb(out["nuc"][0][1], bg_label=0)) # type_map

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Dataset Details

Semi-manually annotated CIN2 samples from a (private) cohort of Helsinki University Hospital

Contains:

• 370 varying sized image crops at 20x magnification.
• 168 640 annotated nuclei

Dataset classes

nuc_classes = {
    0: "background",
    1: "neoplastic",
    2: "inflammatory",
    3: "connective",
    4: "dead",
    5: "glandular_epithelial",
    6: "squamous_epithelial",
}

Dataset Class Distribution

• connective nuclei: 46 222 (~27.3%)
• neoplastic nuclei: 49 493 (~29.4%)
• inflammatory nuclei 27 226 (~16.1%)
• dead nuclei 195 (~0.11%)
• glandular epithelial 14 310 (~8.5%)
• squamous epithelial 31194 (~18.5%)

Model Training Details:

First, the image crops in the training data were tiled into 224x224px patches with a sliding window (stride=32px).

Rest of the training procedures follow this notebook: [link]

Citation

cellseg_models.pytorch:

@misc{https://doi.org/10.5281/zenodo.12666959,
  doi = {10.5281/ZENODO.12666959},
  url = {https://zenodo.org/doi/10.5281/zenodo.12666959},
  author = {Okunator,  },
  title = {okunator/cellseg_models.pytorch: v0.2.0},
  publisher = {Zenodo},
  year = {2024},
  copyright = {Creative Commons Attribution 4.0 International}
}

Hover-Net original paper:

@article{Graham2019,
  title = {Hover-Net: Simultaneous segmentation and classification of nuclei in multi-tissue histology images},
  volume = {58},
  ISSN = {1361-8415},
  url = {http://dx.doi.org/10.1016/j.media.2019.101563},
  DOI = {10.1016/j.media.2019.101563},
  journal = {Medical Image Analysis},
  publisher = {Elsevier BV},
  author = {Graham,  Simon and Vu,  Quoc Dang and Raza,  Shan E Ahmed and Azam,  Ayesha and Tsang,  Yee Wah and Kwak,  Jin Tae and Rajpoot,  Nasir},
  year = {2019},
  month = dec,
  pages = {101563}
}

Licence

These model weights are released under the Apache License, Version 2.0 (the "License"). You may obtain a copy of the License at:

http://www.apache.org/licenses/LICENSE-2.0

Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.

Additional Terms

While the Apache 2.0 License grants broad permissions, we kindly request that users adhere to the following guidelines: Medical or Clinical Use: This model is not intended for use in medical diagnosis, treatment, or prevention of disease of real patients. It should not be used as a substitute for professional medical advice.